Soluble biospecific macromolecule for purification of estrogen receptor.
نویسندگان
چکیده
منابع مشابه
Soluble biospecific macromolecule for purification of estrogen receptor.
An alternative to affinity chromatography purification of proteins based on the use of a water-soluble biospecific polymer has been applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (molecular weight approximately 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released ...
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Soluble Estrogen Receptor Monoclonal Antibodies against a Component Related to
Mice were immunized with 1 to 3 fig of cytoplasmic estrogen receptor fragment purified from human myometrium by affinity chromatography. Two RE-antibody-secreting clones were de tected from one fusion that were capable of precipitating cytosol RE. Monoclonal antibody D5 (subclass Igd) reacts with an antigen that is related to RE from immunoprecipitation studies but which can be separated from t...
متن کاملMonoclonal antibodies against a component related to soluble estrogen receptor.
Mice were immunized with 1 to 3 micrograms of cytoplasmic estrogen receptor fragment purified from human myometrium by affinity chromatography. Two RE-antibody-secreting clones were detected from one fusion that were capable of precipitating cytosol RE. Monoclonal antibody D5 (subclass IgG1) reacts with an antigen that is related to RE from immunoprecipitation studies but which can be separated...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.7.3143